SYBR Green
SYBR Green (Ex-Max 495 nm/Em-Max 518 nm)
SYBR Green I (Thiazole Green) is a dsDNA selective nucleic acid stain that is often used in qPCR and as a DNA gel stain. It is excited at 495 nm and emits at 518 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 495 nm
- Emission maximum
- 518 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Molecular weight
- 509.73
SYBR Green has an excitation maximum at 495 nm and an emission maximum at 518 nm, a Stokes shift of 23 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is SYBR Green?
- SYBR Green I (Thiazole Green) is a dsDNA selective nucleic acid stain that is often used in qPCR and as a DNA gel stain. It is excited at 495 nm and emits at 518 nm.
- What laser excites SYBR Green?
- SYBR Green is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
- What filter should I use to detect SYBR Green?
- A 515/30 filter captures the peak of the SYBR Green emission near 518 nm with minimal spillover.