FVS520
FVS520 (Ex-Max 490 nm/Em-Max 515 nm)
FVS520 (Fixable Viability Stain 520) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 490 nm and an emission peak at 515 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Commercial antibodies
- 1 from 1 supplier
FVS520 has an excitation maximum at 490 nm and an emission maximum at 515 nm, a Stokes shift of 25 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is FVS520?
- FVS520 (Fixable Viability Stain 520) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 490 nm and an emission peak at 515 nm.
- What laser excites FVS520?
- FVS520 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect FVS520?
- A 515/30 filter captures the peak of the FVS520 emission near 515 nm with minimal spillover.
- How many antibodies are available conjugated to FVS520?
- FluoroFinder tracks 1 commercially available FVS520 conjugates, searchable by target, clone, host and application.