Calcein Green
Calcein Green (Ex-Max 493 nm/Em-Max 512 nm)
Calcein Green is a green fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Green AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Green AM is hydrolyzed by esterase into the fluorescent Calcein Green which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. It has an excitation peak at 493 nm and an emission peak at 512 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 493 nm
- Emission maximum
- 512 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
Calcein Green has an excitation maximum at 493 nm and an emission maximum at 512 nm, a Stokes shift of 19 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Calcein Green?
- Calcein Green is a green fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Green AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Green AM is hydrolyzed by esterase into the fluorescent Calcein Green which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. It has an excitation peak at 493 nm and an emission peak at 512 nm.
- What laser excites Calcein Green?
- Calcein Green is optimally excited by the 488 laser line. Its excitation maximum is 493 nm.
- What filter should I use to detect Calcein Green?
- A 525/50 filter captures the peak of the Calcein Green emission near 512 nm with minimal spillover.