Alexa Fluor 488 Dye Profile


488

Laser Line

530/43

Common Filter

490

Excitation Max

525

Emission Max


Spectra Viewer

Dyes similar to Alexa Fluor 488 based on emission

Acridine Orange ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet CF503R CFSE Chromeo 488 Chromeo 505 Citrine FITC Plus CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 GelGreen GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoSensor Yellow/Blue pH9.0 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine mCitrine MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 STAR 512 StarBright Blue 520 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright FITC VioGreen Vybrant DyeCycle Green YFP ZsGreen

About Alexa Fluor 488


Alexa Fluor® 488 (AF488, Alexa 488) is a green-emitting synthetic fluorophore that can be excited by the 488 nm blue laser and captured with a 530/30 nm bandpass filter. AF488 has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. AF488 is one of the most popular Alexa Fluor dyes and is widely used in fluorescent microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.


Popular Alexa Fluor 488 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
R&D Systems
FAB937G-100UG
FC CD137
Mouse
Rat
IgG2a
158332
100 μg
$237
View
R&D Systems
FAB9929G-100UG
FC CD3
Human
Human
IgG1
Hu113
100 μg
$384
View
Novus Biologicals
NBP2-22119AF488
FC,ELISA,IHC-P,WB,IHC iNOS
Human,Mouse
Mouse
IgG1
400000
0.1 ml
$485
View
R&D Systems
FAB72271G
FC CD34
Human
Mouse
IgG1
756510
100 Tests
$341
View
Novus Biologicals
NB600-562AF488
ICC, IF,FC,IHC-P,IHC-Fr,WB,IHC CD31
Human,Feline,Rabbit,Mouse
Mouse
IgG1 κ
JC/70A
0.1 ml
$485
View
R&D Systems
FAB2188G-100UG
FC Carbonic Anhydrase IX
Human
Mouse
IgG2a
303123
100 μg
$421
View
Sony
1149080
IF,FC,IHC CD45.2
Mouse
Mouse
IgG2a κ
104
100 μg
$226
View
R&D Systems
FAB3715G-025
FC Fibroblast Activation Protein alpha
Human
Mouse
IgG1
427819
25 Tests
$205
View
R&D Systems
FAB1217G
FC,ICC CD45R (B220)
Mouse
Rat
IgG2a
RA3-6B2
100 Tests
$341
View
Novus Biologicals
NBP2-53131AF488
FC Ly-6G
Mouse
Rat
IgG2a κ
1A8
0.1 ml
$356
View
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Popular Alexa Fluor 488 Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
348216
FC IgD
Human
Mouse
IgG2a κ
IA6-2
100 Tests
$265
View
BioLegend
405718
FC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
100 μg
$265
View
BioLegend
406522
FC IgM
Mouse
Rat
IgG2a κ
RMM-1
100 μg
$229
View
BioLegend
406521
FC IgM
Mouse
Rat
IgG2a κ
RMM-1
25 μg
$89
View
BioLegend
405319
FC,IHC-Fr IgG
Mouse
Goat
IgG
Poly4053
100 μg
$183
View
BioLegend
348215
FC IgD
Human
Mouse
IgG2a κ
IA6-2
25 Tests
$118
View
BioLegend
314534
FC IgM
Human,African Green Monkey,Baboon,Cynomolgus,Rhesus
Mouse
IgG1 κ
MHM-88
100 Tests
$347
View
Sony
2632610
FC IgM
Mouse
Rat
IgG2a κ
RMM-1
100 μg
$226
View
Thermo Fisher Scientific
A10631
IF,FC IgG1
Human
Mouse
IgG1
Polyclonal
250 μl
$139
View
BioLegend
406416
FC IgG
Rabbit
Donkey
Ig
Poly4064
100 μg
$141
View
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Alexa Fluor 488 Conjugation Kits


Product Type Company/SKU Applications Size / Price Details
Conjugation Kit Expedeon
332-0030
Labeling 3 x 20 μg
$332
View
Conjugation Kit Expedeon
332-0010
Labeling 3 x 200 μg
$475
View
Conjugation Kit Expedeon
332-0015
Labeling 1 x 2 mg
$803
View
Conjugation Kit Expedeon
332-0005
Labeling 1 x 200 μg
$269
View
Conjugation Kit Thermo Fisher Scientific
A20181
Labeling 5 x 100 μg
$484
View

Alexa Fluor 488 Conjugation Services


Product Type Company/SKU Applications Details
Conjugation Service BioLegend
BL-CONJ-AF488
N/A View
Conjugation Service Columbia Biosciences
COLUMBIA-CONJ-Alexa Fluor 488
Labeling View
Conjugation Service BD Biosciences
BD-CONJ-AF488
N/A View
Conjugation Service Biotium
BIOTIUM-CONJ-AF488
N/A View