About Alexa Fluor 488

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Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A.

Alternative Dyes to Alexa Fluor 488

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SureLight 488 NL 493 Oyster 488 PromoFluor 488P KIRAVIA Blue 520 CF488A STAR 488 VRDye 490 Fluorescein Chromeo 505 CoraLite Plus 488 MaxLight 490 Chromeo 488 Rhodamine 110 Oregon Green 488 FITC ATTO 488

Dyes similar to Alexa Fluor 488 based on emission

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5-CFDA 5-FAM Acridine Orange Aldefluor ATTO 488 BactoView Live Green Brilliant Blue 515 BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BDY FL SE BDY FL-X SE BODIPY 500/510 Brilliant Violet 510 Calcein AM Calcein Calcein Green Calcium Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace CFSE CellTrace Oregon Green CellTracker Violet CF440 CF488A CF500 CFSE Chromeo 488 Chromeo 505 Citrine CoraLite Plus 488 FITC Plus CPN510 Green CytoTrace Green CMFDA CytoTrack Green DCF DMAO DyLight 488 eFluor 514 Calcium Sensor eFluor 520 Fix Viability EFLUXX-ID Green ExoBrite 490/515 EV Membrane Stain eYFP FDG FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS520 GelGreen GFP Ghost Dye Blue 516 HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoBrite Green LysoTracker Green LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoView Green MultiDot 525 NL 493 NovaFluor Blue 510 NucSpot Live 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine Green ROS-ID Green Seta-385-NHS Spark Blue 515 Spectrum Green STAR 440L STAR 440SXP STAR 488 StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Topaz T-Sapphire TSA Vivid 520 Venus Viafluor CFSE Vio 515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright 515 Vio Bright FITC Vio Green VivaFix 408/512 VivaFix 498/521 VRDye 490 YFP Zombie Aqua Zombie Green ZsGreen Aluora 488 RayBright Blue 488 Mpro Tracer CytoLiner 492/510 XFD488 Endoplasmic Reticulum Probe Green Helix NP Green RayBright LIVE 488 CoraLite 488 ATTO 495 ThiolTracker Violet AbBy Fluor 488 cFluor B515 ABflo 488 NucSpot 500/515 AccuGreen HS BDY FL Staurosporine