Alexa Fluor 488 Dye Profile
488

530/43

490

525

Dyes similar to Alexa Fluor 488 based on emission
Acridine Orange ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet CF503R CFSE Chromeo 488 Chromeo 505 Citrine FITC Plus CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 GelGreen GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoSensor Yellow/Blue pH9.0 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine mCitrine MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 STAR 512 StarBright Blue 520 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright FITC VioGreen Vybrant DyeCycle Green YFP ZsGreenAbout Alexa Fluor 488
Alexa Fluor® 488 (AF488, Alexa 488) is a green-emitting synthetic fluorophore that can be excited by the 488 nm blue laser and captured with a 530/30 nm bandpass filter. AF488 has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. AF488 is one of the most popular Alexa Fluor dyes and is widely used in fluorescent microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.
Popular Alexa Fluor 488 Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
R&D Systems FAB937G-100UG |
FC | CD137 Mouse |
Rat IgG2a 158332 |
100 μg $237 |
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R&D Systems FAB9929G-100UG |
FC | CD3 Human |
Human IgG1 Hu113 |
100 μg $384 |
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Novus Biologicals NBP2-22119AF488 |
FC,ELISA,IHC-P,WB,IHC | iNOS Human,Mouse |
Mouse IgG1 400000 |
0.1 ml $485 |
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R&D Systems FAB72271G |
FC | CD34 Human |
Mouse IgG1 756510 |
100 Tests $341 |
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Novus Biologicals NB600-562AF488 |
ICC, IF,FC,IHC-P,IHC-Fr,WB,IHC | CD31 Human,Feline,Rabbit,Mouse |
Mouse IgG1 κ JC/70A |
0.1 ml $485 |
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R&D Systems FAB2188G-100UG |
FC | Carbonic Anhydrase IX Human |
Mouse IgG2a 303123 |
100 μg $421 |
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Sony 1149080 |
IF,FC,IHC | CD45.2 Mouse |
Mouse IgG2a κ 104 |
100 μg $226 |
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R&D Systems FAB3715G-025 |
FC | Fibroblast Activation Protein alpha Human |
Mouse IgG1 427819 |
25 Tests $205 |
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R&D Systems FAB1217G |
FC,ICC | CD45R (B220) Mouse |
Rat IgG2a RA3-6B2 |
100 Tests $341 |
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Novus Biologicals NBP2-53131AF488 |
FC | Ly-6G Mouse |
Rat IgG2a κ 1A8 |
0.1 ml $356 |
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Popular Alexa Fluor 488 Secondary Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
BioLegend 348216 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
100 Tests $265 |
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BioLegend 405718 |
FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
100 μg $265 |
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BioLegend 406522 |
FC | IgM Mouse |
Rat IgG2a κ RMM-1 |
100 μg $229 |
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BioLegend 406521 |
FC | IgM Mouse |
Rat IgG2a κ RMM-1 |
25 μg $89 |
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BioLegend 405319 |
FC,IHC-Fr | IgG Mouse |
Goat IgG Poly4053 |
100 μg $183 |
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BioLegend 348215 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
25 Tests $118 |
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BioLegend 314534 |
FC | IgM Human,African Green Monkey,Baboon,Cynomolgus,Rhesus |
Mouse IgG1 κ MHM-88 |
100 Tests $347 |
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Sony 2632610 |
FC | IgM Mouse |
Rat IgG2a κ RMM-1 |
100 μg $226 |
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Thermo Fisher Scientific A10631 |
IF,FC | IgG1 Human |
Mouse IgG1 Polyclonal |
250 μl $139 |
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BioLegend 406416 |
FC | IgG Rabbit |
Donkey Ig Poly4064 |
100 μg $141 |
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Alexa Fluor 488 Conjugation Kits
Product Type | Company/SKU | Applications | Size / Price | Details |
---|---|---|---|---|
Conjugation Kit |
Expedeon 332-0030 |
Labeling | 3 x 20 μg $332 |
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Conjugation Kit |
Expedeon 332-0010 |
Labeling | 3 x 200 μg $475 |
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Conjugation Kit |
Expedeon 332-0015 |
Labeling | 1 x 2 mg $803 |
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Conjugation Kit |
Expedeon 332-0005 |
Labeling | 1 x 200 μg $269 |
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Conjugation Kit |
Thermo Fisher Scientific A20181 |
Labeling | 5 x 100 μg $484 |
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