488

Laser Line

530/43

Common Filter

490

Excitation Max

525

Emission Max

33,353

Antibodies

5

Conjugation Kits

5

Services


Spectra Viewer

Dyes similar to Alexa Fluor 488 based on emission

Acridine Orange ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet CF503R CFSE Chromeo 488 Chromeo 505 Citrine CoraLite +Fluorescein CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 GelGreen GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoSensor Yellow/Blue pH9.0 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine mCitrine MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 STAR 512 StarBright Blue 520 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright FITC VioGreen Vybrant DyeCycle Green YFP ZsGreen

About Alexa Fluor 488


Alexa Fluor® 488 (AF488, Alexa 488) is a green-emitting synthetic fluorophore that can be excited by the 488 nm blue laser and captured with a 530/30 nm bandpass filter. AF488 has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. AF488 is one of the most popular Alexa Fluor dyes and is widely used in fluorescent microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.


Popular Alexa Fluor 488 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
101217
FC,IHC-Fr,IHC-Fr,3D-IHC CD11b
Mouse,Human,Chimpanzee,Baboon,Cynomolgus,Rhesus,Rabbit
Rat
IgG2b κ
M1/70
100 μg
$215
View
BioLegend
103121
FC CD45
Mouse
Rat
IgG2b κ
30-F11
25 μg
$94
View
BioLegend
302019
FC CD16
Human,African Green Monkey,Baboon,Capuchin Monkey,Chimpanzee,Cynomolgus,Marmoset,Pigtailed Macaque,Rhesus,Non-Human Primate,Squirrel Monkey
Mouse
IgG1 κ
3G8
100 Tests
$248
View
BioLegend
320012
ICS FoxP3
Human,Rat,Mouse,Cynomolgus,Rhesus,Baboon
Mouse
IgG1 κ
150D
100 Tests
$347
View
BioLegend
100210
FC,IHC-Fr,3D-IHC CD3
Mouse
Rat
IgG2b κ
17A2
100 μg
$215
View
BioLegend
100423
FC,IHC-Fr,ICC CD4
Mouse
Rat
IgG2b κ
GK1.5
100 μg
$215
View
BioLegend
100723
FC,3D-IHC CD8a
Mouse
Rat
IgG2a κ
53-6.7
100 μg
$72
View
BioLegend
103122
FC CD45
Mouse
Rat
IgG2b κ
30-F11
100 μg
$215
View
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