CellBrite Fix 488 Dye Profile

Biotium™

488

Laser Line

515/30

Common Filter

480

Excitation Max

513

Emission Max

About CellBrite Fix 488


Biotium's CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium's MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.



Spectra



Dyes similar to CellBrite Fix 488 based on emission

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5-CFDA 5-FAM AccuClear Acridine Orange Aldefluor Alexa Fluor 488 ATTO 465 BactoView Live Green Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Calcein Green Carboxyfluorescein CellBrite Green CellROX Green CellTrace CFSE CellTracker Violet CF440 CF488A CF500 CFSE Chromeo 488 CoraLite Plus 488 FITC Plus CPN510 Green Cy2 CytoTrace Green CMFDA CytoTrack Green DiO DY-380XL DyLight 488 eFluor 506 eFluor 514 Calcium Sensor eFluor 520 Fix Viability EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG Flash Phalloidin Green 488 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS510 FVS520 GFP Ghost Dye Blue 516 Ghost Dye Violet 510 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Live/Dead Fix Lime Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 Mag-Fura-2 mAmetrine MaxLight 490 mAzamiGreen MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 Neuro DiO NeuroVue Jade NL 493 NovaFluor Blue 510 Nuclear Yellow NucSpot Live 488 Oregon Green 488 BAPTA Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 Seta-385-NHS Spark Blue 515 Spectrum Green STAR 440L STAR 440SXP StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green TFAX 488 SE TFAX 488 TFP Thiazole Green T-Sapphire TSA Vivid 520 Viafluor CFSE Vio 515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright 515 Vio Bright FITC Vio Green VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen NucSpot 500/515 AbBy Fluor 488 BactoSpore 485/500 Membrane Stain cFluor B515 Tricyclic cytosine tC RayBright Blue 488 ABflo 488 BDY FL Staurosporine XFD488 Endoplasmic Reticulum Probe Green Aluora 488 Lysosome Probe Green Mpro Tracer CytoLiner 492/510 Helix NP Green BDY Lenalidomide