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EvaGreen Plus
EvaGreen Plus (Ex-Max 487 nm/Em-Max 529 nm)
EvaGreen Plus from Biotium is a fluorogenic nucleic acid stain often used for in-gel detection of DNA and qPCR amplification. EvaGreen Plus has improved signal to noise as compared to the EvaGreen reagent. EvaGreen is compatible with qPCR, HRM and LAMP.
Excitation & emission spectra
Key facts
- Excitation maximum
- 487 nm
- Emission maximum
- 529 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
EvaGreen Plus has an excitation maximum at 487 nm and an emission maximum at 529 nm, a Stokes shift of 42 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is EvaGreen Plus?
- EvaGreen Plus from Biotium is a fluorogenic nucleic acid stain often used for in-gel detection of DNA and qPCR amplification. EvaGreen Plus has improved signal to noise as compared to the EvaGreen reagent. EvaGreen is compatible with qPCR, HRM and LAMP.
- What laser excites EvaGreen Plus?
- EvaGreen Plus is optimally excited by the 488 laser line. Its excitation maximum is 487 nm.
- What filter should I use to detect EvaGreen Plus?
- A 525/50 filter captures the peak of the EvaGreen Plus emission near 529 nm with minimal spillover.