About Live-or-Dye 488/515

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Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 488/515 has an excitation peak at 488 nm and an emission peak at 515 nm.

Alternative Dyes to Live-or-Dye 488/515

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FVS520 Ghost Dye Blue 516 VivaFix 498/521 Zombie Green

Dyes similar to Live-or-Dye 488/515 based on emission

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5-CFDA 5-FAM AccuClear Acridine Orange Aldefluor Alexa Fluor 488 ATTO 465 ATTO 488 BactoView Live Green Brilliant Blue 515 BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace CFSE CellTrace Oregon Green CellTracker Violet CF440 CF488A CF500 CFSE Chromeo 488 CoraLite Plus 488 FITC Plus CPN510 Green Cy2 CytoTrace Green CMFDA CytoTrack Green DCF DY-380XL DyLight 488 eFluor 514 Calcium Sensor eFluor 520 Fix Viability EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG FITC Flash Phalloidin Green 488 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS520 GFP Ghost Dye Blue 516 Ghost Dye Violet 510 HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Live/Dead Fix Aqua Live/Dead Fix Green LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 mAmetrine MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoTracker Green MitoView Green MultiDot 525 NeuroVue Jade NL 493 NovaFluor Blue 510 NucSpot Live 488 Oregon Green 488 BAPTA Oregon Green 488 Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 Rhodamine Green ROS-ID Green Seta-385-NHS Spark Blue 515 Spectrum Green STAR 440L STAR 440SXP STAR 488 StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green T-Sapphire TSA Vivid 520 Viafluor CFSE Vio 515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright 515 Vio Bright FITC Vio Green VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen Mpro Tracer Aluora 488 BDY FL Staurosporine AbBy Fluor 488 ABflo 488 cFluor B515 BDY Lenalidomide XFD488 Helix NP Green AccuGreen HS RayBright Blue 488 Endoplasmic Reticulum Probe Green Lysosome Probe Green NucSpot 500/515 RayBright LIVE 488 CytoLiner 492/510