PhenoVue Fluor 488
PhenoVue™ Fluor 488 (Ex-Max 490 nm/Em-Max 515 nm)
PhenoVue™ Fluor 488 from Perkin Elmer has an excitation peak at 495 nm and an emission peak at 520 nm. PhenoVue™ 488 is an alternative to Alexa Fluor™ 488 and DyLight™ 488. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
PhenoVue Fluor 488 has an excitation maximum at 490 nm and an emission maximum at 515 nm, a Stokes shift of 25 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PhenoVue Fluor 488?
- PhenoVue™ Fluor 488 from Perkin Elmer has an excitation peak at 495 nm and an emission peak at 520 nm. PhenoVue™ 488 is an alternative to Alexa Fluor™ 488 and DyLight™ 488. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
- What laser excites PhenoVue Fluor 488?
- PhenoVue Fluor 488 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect PhenoVue Fluor 488?
- A 515/30 filter captures the peak of the PhenoVue Fluor 488 emission near 515 nm with minimal spillover.
- How bright is PhenoVue Fluor 488?
- PhenoVue Fluor 488 has a relative brightness of 2 out of 5.