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5-FAM
5-FAM (Ex-Max 490 nm/Em-Max 515 nm)
5-FAM (5-Carboxyfluorescein) is a single isomer form of fluorescein, a foundational fluorophore that excites at 490 nm and emits at 515 nm. Carboxyfluorescein is often used in a variety of assays or conjugated to antibodies and detection molecules. FAM is known to be pH sensitive and is often used in to sense pH changes.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Molecular weight
- 376.3
5-FAM has an excitation maximum at 490 nm and an emission maximum at 515 nm, a Stokes shift of 25 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is 5-FAM?
- 5-FAM (5-Carboxyfluorescein) is a single isomer form of fluorescein, a foundational fluorophore that excites at 490 nm and emits at 515 nm. Carboxyfluorescein is often used in a variety of assays or conjugated to antibodies and detection molecules. FAM is known to be pH sensitive and is often used in to sense pH changes.
- What laser excites 5-FAM?
- 5-FAM is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect 5-FAM?
- A 515/30 filter captures the peak of the 5-FAM emission near 515 nm with minimal spillover.