About Calcein AM

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Calcein, AM, also called Calcein Green, AM, is a fluorogenic green fluorescent molecule (ex 491/ em 511) that penetrates the membranes of live cells. Once inside the live cell, Calcein, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.

Dyes similar to Calcein AM based on emission

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5-CFDA 5-FAM AccuClear Aldefluor Alexa Fluor 488 ATTO 465 Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellROX Green CellTrace CFSE CellTracker Violet CF440 CF488A CF500 Chromeo 488 CoraLite Plus 488 FITC Plus CPN510 Green Cy2 CytoTrace Green CMFDA DiO DY-380XL DyLight 488 eFluor 506 eFluor 506 Fix Viability eFluor 514 Calcium Sensor EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG Flash Phalloidin Green 488 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS510 FVS520 GFP Ghost Dye Blue 516 Ghost Dye Violet 510 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Live/Dead Fix Lime Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 Mag-Fura-2 MaxLight 490 mAzamiGreen MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 Neuro DiO NeuroVue Jade NL 493 NovaFluor Blue 510 Nuclear Yellow NucSpot Live 488 Oregon Green 488 BAPTA Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PKH67 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 Seta-385-NHS Spark Blue 515 Spectrum Green STAR 440L STAR 440SXP StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green TFAX 488 SE TFAX 488 TFP T-Sapphire TSA Vivid 520 Viafluor CFSE Vio 515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright 515 Vio Green VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen Helix NP Green BDY FL Staurosporine cFluor B515 BactoSpore 485/500 Membrane Stain XFD488 Aluora 488 Tricyclic cytosine tC Mpro Tracer CytoLiner 492/510 RayBright Blue 488 BDY Lenalidomide Endoplasmic Reticulum Probe Green CoraLite 488 AbBy Fluor 488 Lysosome Probe Green ABflo 488 NucSpot 500/515