Fluo-4
Fluo-4 (Ex-Max 494 nm/Em-Max 516 nm)
Fluo-4, AM a cell-permeant calcium detection reagent. Fluo-4 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 494 nm and an emission peak at 516 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 494 nm
- Emission maximum
- 516 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Extinction coefficient
- 82000
- Molecular weight
- 9247
Fluo-4 has an excitation maximum at 494 nm and an emission maximum at 516 nm, a Stokes shift of 22 nm. Its extinction coefficient is 82000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Fluo-4?
- Fluo-4, AM a cell-permeant calcium detection reagent. Fluo-4 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 494 nm and an emission peak at 516 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
- What laser excites Fluo-4?
- Fluo-4 is optimally excited by the 488 laser line. Its excitation maximum is 494 nm.
- What filter should I use to detect Fluo-4?
- A 515/30 filter captures the peak of the Fluo-4 emission near 516 nm with minimal spillover.