Spark PLUS B488
Spark PLUS B488™ from Biolegend is a fluorophore designed for both conventional and spectral flow cytometry with an excitation peak at 488 nm and an emission peak at 520 nm. This fluorophore is most efficiently excited by the 488 nm laser and offers improved brightness compared to traditional fluorophores like FITC. Its ability to be unmixed from dyes like Spark PLUS B550™, Spark PLUS B574™, and PE, makes it a good option to include in large spectral panels.
Excitation & emission spectra
Key facts
- Excitation maximum
- 493 nm
- Emission maximum
- 516 nm
- Optimal laser line
- 488
- Relative brightness
- 4 of 5
- Commercial conjugates
- 18 from 2 suppliers
Spark PLUS B488 has an excitation maximum at 493 nm and an emission maximum at 516 nm, a Stokes shift of 23 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Spark PLUS B488?
- Spark PLUS B488™ from Biolegend is a fluorophore designed for both conventional and spectral flow cytometry with an excitation peak at 488 nm and an emission peak at 520 nm. This fluorophore is most efficiently excited by the 488 nm laser and offers improved brightness compared to traditional fluorophores like FITC. Its ability to be unmixed from dyes like Spark PLUS B550™, Spark PLUS B574™, and PE, makes it a good option to include in large spectral panels.
- What laser excites Spark PLUS B488?
- Spark PLUS B488 is optimally excited by the 488 laser line. Its excitation maximum is 493 nm.
- How bright is Spark PLUS B488?
- Spark PLUS B488 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to Spark PLUS B488?
- FluoroFinder tracks 18 commercially available Spark PLUS B488 conjugates, searchable by target, clone, host and application.