MemBrite Fix 488/515
MemBrite Fix 488/515 (Ex-Max 490 nm/Em-Max 516 nm)
Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 488/515 has an excitaion peak at 490 nm and an emission peak at 516 nm and are recommended for use in fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 516 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
MemBrite Fix 488/515 has an excitation maximum at 490 nm and an emission maximum at 516 nm, a Stokes shift of 26 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is MemBrite Fix 488/515?
- Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 488/515 has an excitaion peak at 490 nm and an emission peak at 516 nm and are recommended for use in fluorescence microscopy.
- What laser excites MemBrite Fix 488/515?
- MemBrite Fix 488/515 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect MemBrite Fix 488/515?
- A 515/30 filter captures the peak of the MemBrite Fix 488/515 emission near 516 nm with minimal spillover.