Thiazole Green
Thiazole Green (Ex-Max 498 nm/Em-Max 522 nm)
Thiazole Green (SYBR Green I) is a dsDNA selective nucleic acid stain that is often used in qPCR and as a DNA gel stain. It is excited at 495 nm and emits at 518 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 498 nm
- Emission maximum
- 522 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
Thiazole Green has an excitation maximum at 498 nm and an emission maximum at 522 nm, a Stokes shift of 24 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Thiazole Green?
- Thiazole Green (SYBR Green I) is a dsDNA selective nucleic acid stain that is often used in qPCR and as a DNA gel stain. It is excited at 495 nm and emits at 518 nm.
- What laser excites Thiazole Green?
- Thiazole Green is optimally excited by the 488 laser line. Its excitation maximum is 498 nm.
- What filter should I use to detect Thiazole Green?
- A 515/30 filter captures the peak of the Thiazole Green emission near 522 nm with minimal spillover.