YFP
YFP (Ex-Max 514 nm/Em-Max 527 nm)
Yellow fluorescent protein (YFP) is a weakly dimeric yellow-emitting fluorescent protein. It has an excitation peak at 514 nm and an emission peak at 527 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 514 nm
- Emission maximum
- 527 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
YFP has an excitation maximum at 514 nm and an emission maximum at 527 nm, a Stokes shift of 13 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is YFP?
- Yellow fluorescent protein (YFP) is a weakly dimeric yellow-emitting fluorescent protein. It has an excitation peak at 514 nm and an emission peak at 527 nm.
- What laser excites YFP?
- YFP is optimally excited by the 488 laser line. Its excitation maximum is 514 nm.
- What filter should I use to detect YFP?
- A 525/50 filter captures the peak of the YFP emission near 527 nm with minimal spillover.