Oregon Green 514
Oregon Green 514 (Ex-Max 489 nm/Em-Max 526 nm)
Oregon Green 514 is a fluorophore with an excitation peak at 489 nm and an emission peak at 526 nm. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 489 nm
- Emission maximum
- 526 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
Oregon Green 514 has an excitation maximum at 489 nm and an emission maximum at 526 nm, a Stokes shift of 37 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Oregon Green 514?
- Oregon Green 514 is a fluorophore with an excitation peak at 489 nm and an emission peak at 526 nm. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
- What laser excites Oregon Green 514?
- Oregon Green 514 is optimally excited by the 488 laser line. Its excitation maximum is 489 nm.
- What filter should I use to detect Oregon Green 514?
- A 525/50 filter captures the peak of the Oregon Green 514 emission near 526 nm with minimal spillover.