DyLight 488 Dye Profile

Thermo Fisher Scientific™

488

Laser Line

525/50

Common Filter

493

Excitation Max

518

Emission Max

70000

Extinction Coefficient

About DyLight 488


DyLight™ 488 has an excitation peak at 493 nm and an emission peak at 518 nm and is spectrally similar to Alexa Fluor™ 488, fluorescein and FITC. DyLight™ 488 is most commonly used in flow cytometery, and fluorescence microscopy applications.



Spectra



Dyes similar to DyLight 488 based on emission

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5-CFDA 5-FAM Acridine Orange Aldefluor Alexa Fluor 488 ATTO 488 BactoView Live Green Brilliant Blue 515 BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Calcein Green Calcium Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace CFSE CellTrace Oregon Green CellTracker Violet CF440 CF488A CF500 CFSE Chromeo 488 Chromeo 505 CoraLite Plus 488 FITC Plus CPN510 Green CytoTrace Green CMFDA CytoTrack Green DCF eFluor 514 Calcium Sensor eFluor 520 Fix Viability EFLUXX-ID Green Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS520 GFP Ghost Dye Blue 516 HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 mAmetrine MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoTracker Green MitoView Green MultiDot 525 NL 493 NovaFluor Blue 510 NucSpot Live 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine Green ROS-ID Green Seta-385-NHS Spark Blue 515 Spectrum Green STAR 440L STAR 440SXP STAR 488 StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Topaz T-Sapphire TSA Vivid 520 Viafluor CFSE Vio 515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright 515 Vio Bright FITC Vio Green VivaFix 408/512 VivaFix 498/521 VRDye 490 YFP YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen RayBright Blue 488 XFD488 Endoplasmic Reticulum Probe Green Lysosome Probe Green Helix NP Green RayBright LIVE 488 CoraLite 488 ABflo 488 ThiolTracker Violet cFluor B515 BDY FL Staurosporine ATTO 495 AbBy Fluor 488 Aluora 488 Mpro Tracer NucSpot 500/515 CytoLiner 492/510 AccuGreen HS

Popular DyLight 488 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Novus Biologicals, a Bio-Techne brand
NB100-384G
WB,FC,ICC/IF,IHC-P,IHC,Knockout Validated gamma H2AX
Human,Mouse,Rat,Canine
Rabbit
IgG
0.1 ml
$629
View
Thermo Fisher Scientific
MA5-16115
WB,ELISA,IHC-P,IHC,FC Blimp 1
Human,Mouse,Porcine
Mouse
IgG1
3H2-E8
100 μl
$446
View
Novus Biologicals, a Bio-Techne brand
NB110-3638G
WB,ELISA,FC,IHC-Fr,IHC-P,ICC/IF,IHC Alkaline Phosphatase
Human,Mouse,Rat
Mouse
IgG2a κ
8B6
0.1 ml
$499
View
Novus Biologicals, a Bio-Techne brand
NBP3-20834G
IHC-P,IHC-Fr HLA-DR
Human
Rabbit
IgG κ
HLA-DRA/6839R
100 μl
$629
View
Novus Biologicals, a Bio-Techne brand
NB600-985G
WB,FC,IHC-Fr,IHC-P,ICC/IF,IHC CD68
Rat,Primate,Bovine,Monkey,Human,Mouse,Feline
Mouse
IgG1
ED1
0.125 ml
$549
View
Novus Biologicals, a Bio-Techne brand
NBP1-50071G
FC Granzyme B
Human,Primate
Mouse
IgG1
CLB-GB11
0.1 ml
$509
View
Novus Biologicals, a Bio-Techne brand
NBP2-47918G
FC,IHC-Fr CD95
Human
Mouse
IgG1 κ
B-R18
0.1 ml
$519
View
Novus Biologicals, a Bio-Techne brand
NBP2-21789G
IHC-P,WB,FC,IHC-Fr,IHC MHC II
Mouse
Rat
IgG2b
M5/114
0.1 ml
$469
View
Novus Biologicals, a Bio-Techne brand
NBP1-71770G
WB,ELISA,IHC-P,ICC/IF,IHC PAR1
Human,Mouse
Mouse
IgG1
N2-11
0.1 ml
$499
View
Novus Biologicals, a Bio-Techne brand
NB100-64360G
FC CD19
Human
Mouse
IgG1
Bu12
0.1 ml
$509
View
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Popular DyLight 488 Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
406404
FC,ICC IgG
Rabbit
Donkey
Ig
Poly4064
100 μg
$106
View
Bio-Rad
STAR117D488GA
IF,FC IgG
Mouse
Goat
IgG
100 μg
$124
View
Novus Biologicals, a Bio-Techne brand
NBP1-51104G
FC,ELISA IgG2a
Rat
Rat
IgG2a κ
KLH/G2a-1-1
0.25 ml
$319
View
Novus Biologicals, a Bio-Techne brand
NB410-28088G
FC,ELISA,FLISA IgG
Goat
Goat
IgG
0.5 ml
$349
View
Novus Biologicals, a Bio-Techne brand
NBP1-49000G
FC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
0.5 ml
$599
View
BioLegend
405310
FC,ICC IgG
Mouse
Goat
IgG
Poly4053
100 μg
$106
View
Novus Biologicals, a Bio-Techne brand
NBP1-72892
WB,ICC/IF,FLISA IgG2b
Mouse
Rabbit
IgG
0.1 mg
$159
View
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DyLight 488 Conjugation Kits


Product Type Company/SKU Applications Size / Price Details
Conjugation Kit
322-0010
Labeling 3 x 200 μg
$479
View
Conjugation Kit Bio-Rad
LNK221D488
Labeling 3 Conjμgations for 200μg antibody
$544
View
Conjugation Kit Expedeon
322-0010
Labeling 3 x 200 μg
$426
View
Conjugation Kit Expedeon
322-0030
Labeling 3 x 20 μg
$298
View
Conjugation Kit Bio-Rad
LNK223D488
Labeling 3 Conjμgations for 20μg antibody
$388
View
Conjugation Kit
322-0015
Labeling 1 x 2 mg
$779
View
Conjugation Kit Expedeon
322-0015
Labeling 1 x 2 mg
$721
View
Conjugation Kit Expedeon
322-0005
Labeling 1 x 200 μg
$241
View
Conjugation Kit
322-0030
Labeling 3 x 20 μg
$329
View
Conjugation Kit Bio-Rad
LNK222D488
Labeling 1 Conjμgation for 2mg antibody
$913
View

DyLight 488 Conjugation Services


Product Type Company/SKU Applications Details
Conjugation Service Columbia Biosciences
COLUMBIA-CONJ-DyLight 488
Labeling View
Conjugation Service Biotium
BIOTIUM-CONJ-DL488
N/A View