What laser excites Spark Blue 515?

Spark Blue 515 is optimally excited by the 488 laser line. Its excitation maximum is 489 nm.

What filter should I use to detect Spark Blue 515?

A 515/30 filter captures the peak of the Spark Blue 515 emission near 510 nm with minimal spillover.

How bright is Spark Blue 515?

Spark Blue 515 has a relative brightness of 2 out of 5.

How many antibodies are available conjugated to Spark Blue 515?

FluoroFinder tracks 14 commercially available Spark Blue 515 conjugates, searchable by target, clone, host and application.

Spark Blue 515

Spark Blue 515 (Ex-Max 489 nm/Em-Max 510 nm)

Spark Blue™ 515 exhibits peak excitation/emission wavelengths at 506 nm and 516 nm respectively, most closely matching the spectra of cFluor® B515. On conventional cytometers, it can be detected with a 488 nm blue laser and the 530/30 filter set typically used by FITC or Alexa Fluor® 488. On spectral instruments with the proper settings, it is possible to unmix Spark Blue™ 515 from dyes like FITC, Alexa Fluor® 488, and KIRAVIA Blue 520™ as its peak emission is shifted over 10 nm. With a dim brightness, Spark Blue™ 515 is best suited for detecting antigens with medium to high expression levels. It is also stable towards exposure to heat or a variety of commonly used fixative solutions.

Excitation & emission spectra

Key facts

Excitation maximum: 489 nm
Emission maximum: 510 nm
Optimal laser line: 488
Optimal filter: 515/30
Relative brightness: 2 of 5
Commercial conjugates: 14 from 2 suppliers

Spark Blue 515 has an excitation maximum at 489 nm and an emission maximum at 510 nm, a Stokes shift of 21 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is Spark Blue 515?: Spark Blue™ 515 exhibits peak excitation/emission wavelengths at 506 nm and 516 nm respectively, most closely matching the spectra of cFluor® B515. On conventional cytometers, it can be detected with a 488 nm blue laser and the 530/30 filter set typically used by FITC or Alexa Fluor® 488. On spectral instruments with the proper settings, it is possible to unmix Spark Blue™ 515 from dyes like FITC, Alexa Fluor® 488, and KIRAVIA Blue 520™ as its peak emission is shifted over 10 nm. With a dim brightness, Spark Blue™ 515 is best suited for detecting antigens with medium to high expression levels. It is also stable towards exposure to heat or a variety of commonly used fixative solutions.
What laser excites Spark Blue 515?: Spark Blue 515 is optimally excited by the 488 laser line. Its excitation maximum is 489 nm.
What filter should I use to detect Spark Blue 515?: A 515/30 filter captures the peak of the Spark Blue 515 emission near 510 nm with minimal spillover.
How bright is Spark Blue 515?: Spark Blue 515 has a relative brightness of 2 out of 5.
How many antibodies are available conjugated to Spark Blue 515?: FluoroFinder tracks 14 commercially available Spark Blue 515 conjugates, searchable by target, clone, host and application.