Calcein
Calcein (Ex-Max 491 nm/Em-Max 511 nm)
Calcein, also called Calcein Green, is the hydrodlyzed version of Calcein, AM and Calcein Green, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein, AM and Calcein Green, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 491 nm and an emission peak at 511 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 491 nm
- Emission maximum
- 511 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Molecular weight
- 622.5
Calcein has an excitation maximum at 491 nm and an emission maximum at 511 nm, a Stokes shift of 20 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Calcein?
- Calcein, also called Calcein Green, is the hydrodlyzed version of Calcein, AM and Calcein Green, AM. It is commonly used as a cell impermeant, esterase independet control for Calcein, AM and Calcein Green, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 491 nm and an emission peak at 511 nm.
- What laser excites Calcein?
- Calcein is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.
- What filter should I use to detect Calcein?
- A 515/30 filter captures the peak of the Calcein emission near 511 nm with minimal spillover.