488

Laser Line

515/30

Common Filter

491

Excitation Max

511

Emission Max


Spectra Viewer

Dyes similar to Calcein based on emission

5-CFDA 5-FAM AccuClear Aldefluor ATTO 465 BB515 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellROX Green CellTracker Violet CF440 CF488A CF500 Chromeo 488 CoraLite 488 CoraLite +Fluorescein CPN510 Green Cy2 CytoTrace Green CMFDA DiO DY-380XL DyLight 488 eFluor 506 eFluor 506 Fix Viability eFluor 514 Calcium Sensor EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG Flash Phalloidin Green 488 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS510 FVS520 GFP Ghost Dye Blue 516 Ghost Dye Violet 510 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Live/Dead Fix Lime Live/Dead Fix Scarlet Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 Mag-Fura-2 MaxLight 490 mAzamiGreen MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 Neuro DiO NeuroVue Jade NL 493 NovaFluor Blue 510 Nuclear Yellow NucSpot Live 488 Oregon Green 488 BAPTA Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 PKH67 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 ROS-ID Green Seta-385-NHS Spectrum Green STAR 440SXP StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Syto 16 TFAX 488 SE TFAX 488 TFP T-Sapphire Viafluor CFSE Vio515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright 515 VioGreen VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen

About Calcein


Calcein is a green fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein AM is hydrolyzed by esterase into the fluorescent Calcein which can then be measured via fluorescent microscopy, flow cytometer, or fluorescent microplate reader.