T-Sapphire
T-Sapphire (Ex-Max 399 nm/Em-Max 511 nm)
T-Sapphire is a weakly dimeric fluorescent protein with a long Stokes' Shift in that it has an excitation peak at 399 nm and an emission peak at 511 nm. It was originally derived from the jellyfish, Aequorea victoria.
Excitation & emission spectra
Key facts
- Excitation maximum
- 399 nm
- Emission maximum
- 511 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
- Extinction coefficient
- 44000
- Quantum yield
- 0.6
- Molecular weight
- 26900
T-Sapphire has an excitation maximum at 399 nm and an emission maximum at 511 nm, a Stokes shift of 112 nm. Its extinction coefficient is 44000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is T-Sapphire?
- T-Sapphire is a weakly dimeric fluorescent protein with a long Stokes' Shift in that it has an excitation peak at 399 nm and an emission peak at 511 nm. It was originally derived from the jellyfish, Aequorea victoria.
- What laser excites T-Sapphire?
- T-Sapphire is optimally excited by the 405 laser line. Its excitation maximum is 399 nm.
- What filter should I use to detect T-Sapphire?
- A 515/30 filter captures the peak of the T-Sapphire emission near 511 nm with minimal spillover.