Dyes similar to GFP based on emission5-CFDA 5-FAM AccuClear Aldefluor ATTO 465 BB515 BDY FL SE BDY FL-X SE BODIPY 493/503 BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellTracker Violet CF440 CF488A CF500 Chromeo 488 CoraLite 488 CPN510 Green Cy2 CytoTrace Green CMFDA DiO DY-380XL DyLight 488 eFluor 506 eFluor 506 Fix Viability eFluor 514 Calcium Sensor EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS510 FVS520 Ghost Dye Blue 516 Ghost Dye Violet 510 iFluor 488 Kaede (green) KikGR1 (green) Live/Dead Fix Lime Live/Dead Fix Scarlet Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 Mag-Fura-2 MaxLight 490 mAzamiGreen MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 Neuro DiO NeuroVue Jade NL 493 NovaFluor Blue 510 Nuclear Yellow NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Fluor 488 PKH67 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 ROS-ID Green Seta-385-NHS Spectrum Green STAR 440SXP StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green TFAX 488 SE TFAX 488 TFP T-Sapphire Viafluor CFSE Vio515 VioBright 515 VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen
Green Fluorescent Protein (GFP) is a green-emitting fluorescent protein that can be excited by the 488 nm blue laser and captured with a 510/50 bandpass filter. GFP has excitation peaks at 395 and 475 nm and an emission peak at 509 nm. Originally discovered in jellyfish, GFP is commonly included in recombinant DNA vectors to visually confirm or monitor cloned gene expression. While most small fluorescent molecules such as FITC are highly phototoxic, GFP can be safely expressed with essentially no effect on cellular viability or performance. GFP has been mutated to many different versions with improved spectral or structural properties, such (eg. eGFP and sfGFP).