Brilliant Blue 515
BB515 (Ex-Max 484 nm/Em-Max 631 nm)
Horizon Brilliant™ Blue 515 (BB515), exclusive to BD Biosciences, is a brighter alternative to FITC and Alexa Fluor™ 488 in flow cytometry. It has an excitation peak at 490 nm and an emission peak at 515 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 3 of 5
- Commercial antibodies
- 216 from 1 supplier
Brilliant Blue 515 has an excitation maximum at 490 nm and an emission maximum at 515 nm, a Stokes shift of 25 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Brilliant Blue 515?
- Horizon Brilliant™ Blue 515 (BB515), exclusive to BD Biosciences, is a brighter alternative to FITC and Alexa Fluor™ 488 in flow cytometry. It has an excitation peak at 490 nm and an emission peak at 515 nm.
- What laser excites Brilliant Blue 515?
- Brilliant Blue 515 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect Brilliant Blue 515?
- A 525/50 filter captures the peak of the Brilliant Blue 515 emission near 515 nm with minimal spillover.
- How bright is Brilliant Blue 515?
- Brilliant Blue 515 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to Brilliant Blue 515?
- FluoroFinder tracks 216 commercially available Brilliant Blue 515 conjugates, searchable by target, clone, host and application.