BCECF (Red em) pH5.2 Dye Profile


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About BCECF (Red em) pH5.2

BCECF is a pH indicator in its free acid form with a pKa (6.97). This form can be used to construct a standard curve or as a control for the cell-permeant version of the dye, BCECF-AM. At low pH, the reagent is weakly fluorescent but increases in intensity with increasing pH. This probe can be used ratiometrically as the excitation spectrum shifts between 439 nm and 505 nm, pH depending. This probe has, therefor, a primary excitation peak at 505 nm and a secondary peak at 439 nm with an emission peak that remains constant at 524 nm.


Dyes similar to BCECF (Red em) pH5.2 based on emission

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Acridine Orange Alexa Fluor 488 ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BODIPY 500/510 Calcium Green CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet CF503R CFSE Chromeo 488 Chromeo 505 Citrine FITC Plus CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 GelGreen GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange LIVE 510 Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine mCitrine MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 STAR 512 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 TSA Vivid 520 Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright FITC VioGreen Vybrant DyeCycle Green YFP ZsGreen