CF500
CF®500 (Ex-Max 502 nm/Em-Max 511 nm)
CF®500 from Biotium is a fluorophore with an excitation peak at 502 nm and an emission peak at 511 nm. It is spectrally similar to Fluorescein, Alexa Fluor™ 488 and DyLight™ 488. It can be use in fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 502 nm
- Emission maximum
- 511 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
CF500 has an excitation maximum at 502 nm and an emission maximum at 511 nm, a Stokes shift of 9 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CF500?
- CF®500 from Biotium is a fluorophore with an excitation peak at 502 nm and an emission peak at 511 nm. It is spectrally similar to Fluorescein, Alexa Fluor™ 488 and DyLight™ 488. It can be use in fluorescence microscopy and flow cytometry.
- What laser excites CF500?
- CF500 is optimally excited by the 488 laser line. Its excitation maximum is 502 nm.
- What filter should I use to detect CF500?
- A 515/30 filter captures the peak of the CF500 emission near 511 nm with minimal spillover.
- How bright is CF500?
- CF500 has a relative brightness of 2 out of 5.