Live/Dead Fix Aqua
Live/Dead Fix Aqua (Ex-Max 365 nm/Em-Max 524 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Aqua has an excitation peak at 365 nm and an emission peak at 524 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 365 nm
- Emission maximum
- 524 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
- Commercial antibodies
- 3 from 1 supplier
Live/Dead Fix Aqua has an excitation maximum at 365 nm and an emission maximum at 524 nm, a Stokes shift of 159 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Live/Dead Fix Aqua?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Aqua has an excitation peak at 365 nm and an emission peak at 524 nm.
- What laser excites Live/Dead Fix Aqua?
- Live/Dead Fix Aqua is optimally excited by the 405 laser line. Its excitation maximum is 365 nm.
- What filter should I use to detect Live/Dead Fix Aqua?
- A 515/30 filter captures the peak of the Live/Dead Fix Aqua emission near 524 nm with minimal spillover.
- How many antibodies are available conjugated to Live/Dead Fix Aqua?
- FluoroFinder tracks 3 commercially available Live/Dead Fix Aqua conjugates, searchable by target, clone, host and application.