About Live/Dead Fix Aqua

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The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Aqua has an excitation peak at 365 nm and an emission peak at 524 nm.

Alternative Dyes to Live/Dead Fix Aqua

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FVS510 Ghost Dye Violet 510 Zombie Aqua VivaFix 408/512

Dyes similar to Live/Dead Fix Aqua based on emission

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5-FAM Acridine Orange Alexa Fluor 488 ATTO 488 BactoView Live Green Brilliant Blue 515 BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green Carboxyfluorescein CellBrite Steady 488 CellROX Green CellTrace CFSE CellTrace Oregon Green CellTracker Violet CF440 CF488A CF503R CFSE Chromeo 488 Chromeo 505 Citrine CoraLite Plus 488 FITC Plus CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FDG FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 AM Fluo-4 FLUOFORTE FVS520 GelGreen Ghost Dye Blue 516 GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange LIVE 510 Live/Dead Fix Green Live-or-Dye 488/515 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine MaxLight 490 mCitrine MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 440SXP STAR 488 STAR 512 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 TSA Vivid 520 Venus ViaFluor 488 Viafluor CFSE Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright FITC Vio Green VRDye 490 YFP ZsGreen RayBright LIVE 488 Mpro Tracer Aluora 488 ABflo 488 cFluor B515 ATTO 495 ATTO Rho110 AccuGreen HS RayBright Blue 488 AbBy Fluor 488 cFluor B532 XFD488 ThiolTracker Violet Helix NP Green Aluora 514