Chromeo 488
Chromeo 488 (Ex-Max 488 nm/Em-Max 517 nm)
Chromeo® 488 is a bright, green fluorescent label that can be used in place of Fluorescein, FITC, Alexa Fluor™ 488*, and DyLight™ 488*. It has an excitation peak at 488 nm and an emission peak at 517 nm. The dye, which is compatible with filter sets designed for fluorescein, exhibits superior luminescence properties and has been tested in a variety of applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 517 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 73000
Chromeo 488 has an excitation maximum at 488 nm and an emission maximum at 517 nm, a Stokes shift of 29 nm. Its extinction coefficient is 73000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Chromeo 488?
- Chromeo® 488 is a bright, green fluorescent label that can be used in place of Fluorescein, FITC, Alexa Fluor™ 488*, and DyLight™ 488*. It has an excitation peak at 488 nm and an emission peak at 517 nm. The dye, which is compatible with filter sets designed for fluorescein, exhibits superior luminescence properties and has been tested in a variety of applications.
- What laser excites Chromeo 488?
- Chromeo 488 is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect Chromeo 488?
- A 525/50 filter captures the peak of the Chromeo 488 emission near 517 nm with minimal spillover.
- How bright is Chromeo 488?
- Chromeo 488 has a relative brightness of 2 out of 5.