HiLyte 488
HiLyte™ 488 (Ex-Max 497 nm/Em-Max 525 nm)
HiLyte™ Fluor 488 from AnaSpec has an excitation peak at 497 nm and an emission peak at 525 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 525 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 70000
- Quantum yield
- 0.91
HiLyte 488 has an excitation maximum at 497 nm and an emission maximum at 525 nm, a Stokes shift of 28 nm. Its extinction coefficient is 70000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is HiLyte 488?
- HiLyte™ Fluor 488 from AnaSpec has an excitation peak at 497 nm and an emission peak at 525 nm.
- What laser excites HiLyte 488?
- HiLyte 488 is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect HiLyte 488?
- A 525/50 filter captures the peak of the HiLyte 488 emission near 525 nm with minimal spillover.
- How bright is HiLyte 488?
- HiLyte 488 has a relative brightness of 2 out of 5 (quantum yield 0.91).