TFAX 488 TFP
TFAX 488 TFP (Ex-Max 497 nm/Em-Max 518 nm)
TFAX 488 TFP is an amine reactive green fluorescent dye used in flow cytometry, microscopy, and super resolution microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 518 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 3 of 5
- Extinction coefficient
- 73000
- Quantum yield
- 0.92
- Molecular weight
- 884.91
TFAX 488 TFP has an excitation maximum at 497 nm and an emission maximum at 518 nm, a Stokes shift of 21 nm. Its extinction coefficient is 73000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is TFAX 488 TFP?
- TFAX 488 TFP is an amine reactive green fluorescent dye used in flow cytometry, microscopy, and super resolution microscopy applications.
- What laser excites TFAX 488 TFP?
- TFAX 488 TFP is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect TFAX 488 TFP?
- A 515/30 filter captures the peak of the TFAX 488 TFP emission near 518 nm with minimal spillover.
- How bright is TFAX 488 TFP?
- TFAX 488 TFP has a relative brightness of 3 out of 5 (quantum yield 0.92).