About Qdot 525

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Qdot® 525 from Thermo Fisher Scientific is an inorganic nanocrystal with a surface functionalized to allow antibodies and biomolecules to be cross-linked. It is most efficiently excited between 320-405 nm but is capable of excitation across every laser less than its emission peak. It is significantly brighter and more photostable than traditional organic fluorophores, making it still useful for fluorescence microscopy, however its size and solubility issues make it a challenge to use in flow cytometry. In spectral flow cytometry it may still prove useful due to its very unique spectral characteristics

Alternative Dyes to Qdot 525

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V500 ATTO 465 Krome Orange Pacific Green Oregon Green 488 FITC AmCyan

Dyes similar to Qdot 525 based on emission

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Acridine Orange Alexa Fluor 488 ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green CellBrite Steady 488 CellROX Green CellTrace CFSE CellTrace Oregon Green CellTracker Violet CF503R CFSE Chromeo 488 Chromeo 505 Citrine FITC Plus CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 GelGreen GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange LIVE 510 Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine mCitrine MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 STAR 512 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 TSA Vivid 520 Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright FITC Vio Green Vybrant DyeCycle Green YFP ZsGreen Helix NP Green cFluor B532 AccuGreen HS ATTO Rho110 ThiolTracker Violet XFD488 ATTO 495 RayBright Violet 500 ABflo 488 RayBright LIVE 488 Aluora 514 Aluora 488 Mpro Tracer AbBy Fluor 488