eFluor 520 Fix Viability Dye Profile
488
Laser Line
525/50
Common Filter
488
Excitation Max
522
Emission Max
Dyes similar to eFluor 520 Fix Viability based on emission
5-CFDA 5-FAM Acridine Orange Alexa Fluor 488 ATTO 488 BactoView Live Green BB515 BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace Oregon Green CellTracker Violet CF440 CF488A CFSE Chromeo 488 Chromeo 505 Citrine CoraLite Plus 488 FITC Plus CytoTrace Green CMFDA CytoTrack Green DCF DMAO DyLight 488 eFluor 514 Calcium Sensor EFLUXX-ID Green EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FDG FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 AM Fluo-4 FLUOFORTE Fura-2 (Ca2+free) FVS520 GelGreen Ghost Dye Blue 516 GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine MaxLight 490 mCitrine MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NL 493 NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue Fluor 488 Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 StarBright Blue 520 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 Venus Viafluor CFSE Vio515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright 515 VioBright FITC VioGreen VRDye 490 YFP Zombie Green ZsGreenAbout eFluor 520 Fix Viability
eFluor™ 520 (eF520) Fixable Viability is a green-emitting viability dye that can be excited by the 488 nm blue laser and collected using a 530/30 bandpass filter. eFluor 520 has an excitation peak at 488 nm and and emission peak at 522 nm. eF520 irreversibly stains dead cells with a robust fluorescence that is unaffected by washing, fixation, permeability treatments, or other staining. It is most commonly used for flow cytometry and is spectrally similar to FITC. This eFluor dye is unique in the sense that eFluor 520 is only associated with two products, this product and a phalloidin conjugate. This dye is part of the larger eFluor dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
