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Zombie Aqua

Zombie Aqua (Ex-Max 380 nm/Em-Max 512 nm)

Zombie Aqua from Biolegend is an amine-reactive viability dye useful in flow cytometry when fixation and permeabilization of the cells is required prior to analysis. It has an excitation peak at 380 nm and an emission peak at 512 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
380 nm
Emission maximum
512 nm
Optimal laser line
405
Optimal filter
515/30

Zombie Aqua has an excitation maximum at 380 nm and an emission maximum at 512 nm, a Stokes shift of 132 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is Zombie Aqua?
Zombie Aqua from Biolegend is an amine-reactive viability dye useful in flow cytometry when fixation and permeabilization of the cells is required prior to analysis. It has an excitation peak at 380 nm and an emission peak at 512 nm.
What laser excites Zombie Aqua?
Zombie Aqua is optimally excited by the 405 laser line. Its excitation maximum is 380 nm.
What filter should I use to detect Zombie Aqua?
A 515/30 filter captures the peak of the Zombie Aqua emission near 512 nm with minimal spillover.