Loading the Dye Directory…

FITC Plus

CoraLite +Fluorescein (Ex-Max 495 nm/Em-Max 520 nm)

CoraLite Plus Fluorescein has an excitation peak at 495 nm and an emission peak at 520 nm.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
495 nm
Emission maximum
520 nm
Optimal laser line
488
Optimal filter
515/30
Relative brightness
1 of 5
Commercial conjugates
131 from 1 supplier

FITC Plus has an excitation maximum at 495 nm and an emission maximum at 520 nm, a Stokes shift of 25 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is FITC Plus?
CoraLite Plus Fluorescein has an excitation peak at 495 nm and an emission peak at 520 nm.
What laser excites FITC Plus?
FITC Plus is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
What filter should I use to detect FITC Plus?
A 515/30 filter captures the peak of the FITC Plus emission near 520 nm with minimal spillover.
How bright is FITC Plus?
FITC Plus has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to FITC Plus?
FluoroFinder tracks 131 commercially available FITC Plus conjugates, searchable by target, clone, host and application.