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PhenoVue Cal-520 AM, Calcium Indicator

PhenoVue Cal-520 AM, Calcium Indicator (Ex-Max 493 nm/Em-Max 515 nm)

PhenoVue™ Cal-520 AM is a cell permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Cal-520 AM Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 493 nm and a maximum emission wavelength of 515 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
493 nm
Emission maximum
515 nm
Optimal laser line
488

PhenoVue Cal-520 AM, Calcium Indicator has an excitation maximum at 493 nm and an emission maximum at 515 nm, a Stokes shift of 22 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is PhenoVue Cal-520 AM, Calcium Indicator?
PhenoVue™ Cal-520 AM is a cell permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Cal-520 AM Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 493 nm and a maximum emission wavelength of 515 nm.
What laser excites PhenoVue Cal-520 AM, Calcium Indicator?
PhenoVue Cal-520 AM, Calcium Indicator is optimally excited by the 488 laser line. Its excitation maximum is 493 nm.