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SureLight 488
SureLight 488 (Ex-Max 495 nm/Em-Max 518 nm)
SureLight 488 from Columbia Biosciences is a fluorophore with an excitation peak at 496 nm and an emission peak at 517 nm. SureLight 488 is available in antibody conjugation kits for flow cytometry and fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 496 nm
- Emission maximum
- 517 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
- Commercial antibodies
- 1 from 1 supplier
SureLight 488 has an excitation maximum at 496 nm and an emission maximum at 517 nm, a Stokes shift of 21 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is SureLight 488?
- SureLight 488 from Columbia Biosciences is a fluorophore with an excitation peak at 496 nm and an emission peak at 517 nm. SureLight 488 is available in antibody conjugation kits for flow cytometry and fluorescence microscopy.
- What laser excites SureLight 488?
- SureLight 488 is optimally excited by the 488 laser line. Its excitation maximum is 496 nm.
- What filter should I use to detect SureLight 488?
- A 515/30 filter captures the peak of the SureLight 488 emission near 517 nm with minimal spillover.
- How bright is SureLight 488?
- SureLight 488 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to SureLight 488?
- FluoroFinder tracks 1 commercially available SureLight 488 conjugates, searchable by target, clone, host and application.