About Fura-2 (Ca2+free)

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Fura-2, AM is a cell-permeant ratiometric calcium indicator. When Fura-2, AM loads a cell and is intracellularly hydrolyzed, it exhibits an increase in excitation efficiency at 336nm but not at 368nm. This increases the emission intensity monitored at ~510nm when bound to calcium while excited by 336nm energy while the reduced intensity can be monitored at the ~510nm emission off the 368nm excitation. Because of the unique spectral characteristics, this probe is most commonly used in fluorescence microscopy.

Dyes similar to Fura-2 (Ca2+free) based on emission

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5-CFDA 5-FAM AccuClear Aldefluor Alexa Fluor 488 ATTO 465 Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 493/503 BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellTrace CFSE CellTracker Violet CF440 CF488A CF500 Chromeo 488 CoraLite Plus 488 CPN510 Green Cy2 CytoTrace Green CMFDA DiO DY-380XL DyLight 488 eFluor 506 eFluor 506 Fix Viability eFluor 514 Calcium Sensor EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) FVS510 FVS520 GFP Ghost Dye Blue 516 Ghost Dye Violet 510 iFluor 488 Kaede (green) KikGR1 (green) Live/Dead Fix Lime Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 Mag-Fura-2 MaxLight 490 mAzamiGreen MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MultiDot 525 Neuro DiO NeuroVue Jade NL 493 NovaFluor Blue 510 Nuclear Yellow NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PKH67 PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Seta-385-NHS Spark Blue 515 Spectrum Green STAR 440L STAR 440SXP StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green TFAX 488 SE TFAX 488 TFP T-Sapphire TSA Vivid 520 Viafluor CFSE Vio 515 Vio Bright 515 VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen BactoSpore 485/500 Membrane Stain BDY Lenalidomide Helix NP Green Tricyclic cytosine tC RayBright Blue 488 Lysosome Probe Green ABflo 488 Endoplasmic Reticulum Probe Green ABflo 500 BDY FL Staurosporine Aluora 488 Mpro Tracer AbBy Fluor 488 CytoLiner 492/510 NucSpot 500/515 cFluor B515