Fluo-3 AM Dye Profile


Laser Line


Common Filter


Excitation Max


Emission Max

About Fluo-3 AM

Fluo-3, AM a cell-permeant calcium detection reagent. Fluo-3 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 506 nm and an emission peak at 527 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.


Alternative Dyes to Fluo-3 AM

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Fluo-3 Fluo-4 Calcium Green

Dyes similar to Fluo-3 AM based on emission

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Acridine Orange Alexa Fluor 488 Amethyst Orange ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green CellBrite Steady 488 CellROX Green CellTrace Oregon Green CF503R CFSE Chromeo 488 Chromeo 505 Citrine FITC Plus CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus eYFP FITC Flash Phalloidin Green 488 Fluo-3 GelGreen GOLGI-ID Green HiLyte 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange LIVE 510 Live/Dead Fix Aqua Live/Dead Fix Green LysoView 488 MagDot 525 mAmetrine mCitrine MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 STAR 512 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 TSA Vivid 520 Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye VioBright FITC VioGreen Vybrant DyeCycle Green YFP