Fluo-3 AM
Fluo-3 AM (Ex-Max 504 nm/Em-Max 526 nm)
Fluo-3, AM a cell-permeant calcium detection reagent. Fluo-3 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 506 nm and an emission peak at 527 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 504 nm
- Emission maximum
- 526 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
Fluo-3 AM has an excitation maximum at 504 nm and an emission maximum at 526 nm, a Stokes shift of 22 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Fluo-3 AM?
- Fluo-3, AM a cell-permeant calcium detection reagent. Fluo-3 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 506 nm and an emission peak at 527 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
- What laser excites Fluo-3 AM?
- Fluo-3 AM is optimally excited by the 488 laser line. Its excitation maximum is 504 nm.
- What filter should I use to detect Fluo-3 AM?
- A 525/50 filter captures the peak of the Fluo-3 AM emission near 526 nm with minimal spillover.