VivaFix 498/521
VivaFix 498/521 (Ex-Max 498 nm/Em-Max 512 nm)
VivaFix 498/521 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 498 nm and an emission peak at 521 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 498 nm
- Emission maximum
- 512 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Commercial antibodies
- 1 from 1 supplier
VivaFix 498/521 has an excitation maximum at 498 nm and an emission maximum at 512 nm, a Stokes shift of 14 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is VivaFix 498/521?
- VivaFix 498/521 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 498 nm and an emission peak at 521 nm.
- What laser excites VivaFix 498/521?
- VivaFix 498/521 is optimally excited by the 488 laser line. Its excitation maximum is 498 nm.
- What filter should I use to detect VivaFix 498/521?
- A 515/30 filter captures the peak of the VivaFix 498/521 emission near 512 nm with minimal spillover.
- How many antibodies are available conjugated to VivaFix 498/521?
- FluoroFinder tracks 1 commercially available VivaFix 498/521 conjugates, searchable by target, clone, host and application.