About CFSE

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Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a green-emitting amine-reactive dye that can be excited by the 488 nm blue laser and captured using a 530/43 bandpass filter. It has excitation peak at 494 nm and and an emission peak at 521 nm. CFSE is most commonly used for in vivo cell tracking and proliferation studies. When a cell reproduces, the dye is distributed evenly to daughter cells, with fluorescence intensity halving with each successive passage. This is a generic dye and it can be found in kits from various suppliers.

Alternative Dyes to CFSE

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CFSE CytoTrack Green

Dyes similar to CFSE based on emission

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5-CFDA 5-FAM Acridine Orange Aldefluor Alexa Fluor 488 ATTO 488 BactoView Live Green Brilliant Blue 515 BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcein Green Calcium Green Carboxyfluorescein CellBrite Fix 488 CellBrite Steady 488 CellROX Green CellTrace CFSE CellTrace Oregon Green CellTracker Violet CF440 CF488A Chromeo 488 Chromeo 505 Citrine CoraLite Plus 488 FITC Plus CytoTrace Green CMFDA CytoTrack Green DCF DMAO DyLight 488 eFluor 514 Calcium Sensor eFluor 520 Fix Viability EFLUXX-ID Green EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP FDG FITC Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein FVS520 GelGreen Ghost Dye Blue 516 GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange LIVE 510 Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine MaxLight 490 mCitrine MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NL 493 NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine Green Riboflavin ROS-ID Green Seta-385-NHS Spectrum Green STAR 440SXP STAR 488 STAR 512 StarBright Blue 520 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Topaz TSA Vivid 520 Venus Viafluor CFSE Vio 515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright 515 Vio Bright FITC Vio Green VivaFix 408/512 VivaFix 498/521 VRDye 490 YFP Zombie Aqua Zombie Green ZsGreen RayBright LIVE 488 Aluora 514 Mpro Tracer Aluora 488 ABflo 488 Helix NP Green cFluor B532 AbBy Fluor 488 RayBright Blue 488 NucSpot 500/515 AccuGreen HS ATTO 495 XFD488 ThiolTracker Violet cFluor B515