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NucSpot 500/515

NucSpot 500/515 has an excitation peak at 497 nm and an emission peak at 513 nm. NucSpot Nuclear Stains are cell membrane-impermeant, nuclear-specific counterstains. They have minimal fluorescence until they bind to DNA and can be used for no-wash nuclear staining. They can also be used for selective staining of dead cells in unfixed cell cultures for analysis by flow cytometry or fluorescence imaging.

Excitation & emission spectra

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Key facts

Excitation maximum
497 nm
Emission maximum
513 nm
Commercial antibodies
2 from 1 supplier

NucSpot 500/515 has an excitation maximum at 497 nm and an emission maximum at 513 nm, a Stokes shift of 16 nm.

Frequently asked questions

What is NucSpot 500/515?
NucSpot 500/515 has an excitation peak at 497 nm and an emission peak at 513 nm. NucSpot Nuclear Stains are cell membrane-impermeant, nuclear-specific counterstains. They have minimal fluorescence until they bind to DNA and can be used for no-wash nuclear staining. They can also be used for selective staining of dead cells in unfixed cell cultures for analysis by flow cytometry or fluorescence imaging.
How many antibodies are available conjugated to NucSpot 500/515?
FluoroFinder tracks 2 commercially available NucSpot 500/515 conjugates, searchable by target, clone, host and application.