What laser excites NovaFluor Blue 510?

NovaFluor Blue 510 is optimally excited by the 488 laser line. Its excitation maximum is 496 nm.

What filter should I use to detect NovaFluor Blue 510?

A 525/50 filter captures the peak of the NovaFluor Blue 510 emission near 511 nm with minimal spillover.

How bright is NovaFluor Blue 510?

NovaFluor Blue 510 has a relative brightness of 1 out of 5.

How many antibodies are available conjugated to NovaFluor Blue 510?

FluoroFinder tracks 104 commercially available NovaFluor Blue 510 conjugates, searchable by target, clone, host and application.

NovaFluor Blue 510

NovaFluor Blue 510 (Ex-Max 496 nm/Em-Max 511 nm)

The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 510 is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 496 nm and an emission peak at 511 nm.

Excitation & emission spectra

Key facts

Excitation maximum: 496 nm
Emission maximum: 511 nm
Optimal laser line: 488
Optimal filter: 525/50
Relative brightness: 1 of 5
Commercial antibodies: 104 from 1 supplier

NovaFluor Blue 510 has an excitation maximum at 496 nm and an emission maximum at 511 nm, a Stokes shift of 15 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is NovaFluor Blue 510?: The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Blue 510 is designed to be discreetly excited by the 488 nm laser and has an excitation peak at 496 nm and an emission peak at 511 nm.
What laser excites NovaFluor Blue 510?: NovaFluor Blue 510 is optimally excited by the 488 laser line. Its excitation maximum is 496 nm.
What filter should I use to detect NovaFluor Blue 510?: A 525/50 filter captures the peak of the NovaFluor Blue 510 emission near 511 nm with minimal spillover.
How bright is NovaFluor Blue 510?: NovaFluor Blue 510 has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to NovaFluor Blue 510?: FluoroFinder tracks 104 commercially available NovaFluor Blue 510 conjugates, searchable by target, clone, host and application.