FDG
FDG (Ex-Max 495 nm/Em-Max 515 nm)
FDG, also known as Fluorescein Di-β-D-Galactopyranoside, is a substrate for galactosidases. It has an excitation peak at 495 nm and an emission peak at 515 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 495 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Molecular weight
- 656.6
FDG has an excitation maximum at 495 nm and an emission maximum at 515 nm, a Stokes shift of 20 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is FDG?
- FDG, also known as Fluorescein Di-β-D-Galactopyranoside, is a substrate for galactosidases. It has an excitation peak at 495 nm and an emission peak at 515 nm.
- What laser excites FDG?
- FDG is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
- What filter should I use to detect FDG?
- A 515/30 filter captures the peak of the FDG emission near 515 nm with minimal spillover.