About 5-CFDA

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5-CFDA is a single isomer form of the fluorogenic carboxyfluorescein diacetate (CFDA) often used in live-cell applications to assess cell health and viability. CFDA can also be used as a short-term cell tracker. For long term cell tracking or proliferation assays use instead the CFDA-SE (sometimes errantly abbreviated CFSE) reagent which loads the cell identically but is retained once internalized by the subsequent conjugation of the succinimidyl ester moiety to intracellular amine-containing proteins and peptides.

Alternative Dyes to 5-CFDA

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Calcein Green

Dyes similar to 5-CFDA based on emission

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5-FAM AccuClear Acridine Orange Aldefluor Alexa Fluor 488 ATTO 465 ATTO 488 BactoView Live Green Brilliant Blue 515 BDY FL SE BDY FL-X SE BODIPY 500/510 BODIPY FL Brilliant Violet 510 Calcein AM Calcein Calcein Green Carboxyfluorescein CellBrite Fix 488 CellBrite Green CellROX Green CellTrace CFSE CellTracker Violet CF440 CF488A CF500 CFSE Chromeo 488 CoraLite Plus 488 FITC Plus CPN510 Green Cy2 CytoTrace Green CMFDA CytoTrack Green DY-380XL DyLight 488 eFluor 506 eFluor 514 Calcium Sensor eFluor 520 Fix Viability EFLUXX-ID Green eGFP Emerald GFP ExoBrite 490/515 EV Membrane Stain FDG Flash Phalloidin Green 488 Fluo-4 AM Fluo-4 FLUOFORTE Fluorescein Fura-2 (Ca2+bound) Fura-2 (Ca2+free) FVS510 FVS520 GFP Ghost Dye Blue 516 Ghost Dye Violet 510 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Live/Dead Fix Green Live-or-Dye 488/515 LysoBrite Green LysoTracker Green MagDot 525 Mag-Fluo-4 mAmetrine MaxLight 490 MemBrite Fix 488/515 MemBrite Fix 488/515 MitoLite Green FM MitoTracker Green MitoView Green MultiDot 525 Neuro DiO NeuroVue Jade NL 493 NovaFluor Blue 510 NucSpot Live 488 Oregon Green 488 BAPTA Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue Fura-2 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator PromoFluor 488P ReadiLink 405-508 ReadiLink 492-516 Rhodamine 110 Rhodamine Green Seta-385-NHS Spark Blue 515 Spectrum Green STAR 440L STAR 440SXP StarBright Blue 520 StarBright UltraViolet 510 StarBright Violet 515 STAR GREEN SureLight 488 SYBR Green Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green T-Sapphire TSA Vivid 520 Viafluor CFSE Vio 515 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright 515 Vio Bright FITC Vio Green VivaFix 408/512 VivaFix 498/521 VRDye 490 YO-PRO-1 YOYO-1 Zombie Aqua Zombie Green ZsGreen BDY FL Staurosporine Aluora 488 NucSpot 500/515 Mpro Tracer CytoLiner 492/510 BDY Lenalidomide Tricyclic cytosine tC Lysosome Probe Green Helix NP Green XFD488 cFluor B515 AccuGreen HS RayBright Blue 488 AbBy Fluor 488 Endoplasmic Reticulum Probe Green ABflo 488