Rhodamine Green
Rhodamine Green (Ex-Max 497 nm/Em-Max 523 nm)
Rhodamine Green is a generic fluorophore that has an excitation peak at 497 nm and an emission peak at 523 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 523 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 2 of 5
- Molecular weight
- 430
Rhodamine Green has an excitation maximum at 497 nm and an emission maximum at 523 nm, a Stokes shift of 26 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Rhodamine Green?
- Rhodamine Green is a generic fluorophore that has an excitation peak at 497 nm and an emission peak at 523 nm.
- What laser excites Rhodamine Green?
- Rhodamine Green is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect Rhodamine Green?
- A 525/50 filter captures the peak of the Rhodamine Green emission near 523 nm with minimal spillover.
- How bright is Rhodamine Green?
- Rhodamine Green has a relative brightness of 2 out of 5.