BCECF (525em) pH9.0
BCECF (525em) pH9.0 (Ex-Max 498 nm/Em-Max 524 nm)
BCECF is a pH indicator in its free acid form with a pKa (6.97). This form can be used to construct a standard curve or as a control for the cell-permeant version of the dye, BCECF-AM. At low pH, the reagent is weakly fluorescent but increases in intensity with increasing pH. This probe can be used ratiometrically as the excitation spectrum shifts between 439 nm and 505 nm, pH depending. This probe has, therefor, a primary excitation peak at 505 nm and a secondary peak at 439 nm with an emission peak that remains constant at 524 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 498 nm
- Emission maximum
- 524 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
BCECF (525em) pH9.0 has an excitation maximum at 498 nm and an emission maximum at 524 nm, a Stokes shift of 26 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is BCECF (525em) pH9.0?
- BCECF is a pH indicator in its free acid form with a pKa (6.97). This form can be used to construct a standard curve or as a control for the cell-permeant version of the dye, BCECF-AM. At low pH, the reagent is weakly fluorescent but increases in intensity with increasing pH. This probe can be used ratiometrically as the excitation spectrum shifts between 439 nm and 505 nm, pH depending. This probe has, therefor, a primary excitation peak at 505 nm and a secondary peak at 439 nm with an emission peak that remains constant at 524 nm.
- What laser excites BCECF (525em) pH9.0?
- BCECF (525em) pH9.0 is optimally excited by the 488 laser line. Its excitation maximum is 498 nm.
- What filter should I use to detect BCECF (525em) pH9.0?
- A 525/50 filter captures the peak of the BCECF (525em) pH9.0 emission near 524 nm with minimal spillover.