TSA Vivid 520
TSA Vivid 520 (Ex-Max 494 nm/Em-Max 517 nm)
TSA Vivid™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.
Excitation & emission spectra
Key facts
- Excitation maximum
- 494 nm
- Emission maximum
- 517 nm
- Optimal laser line
- 488
TSA Vivid 520 has an excitation maximum at 494 nm and an emission maximum at 517 nm, a Stokes shift of 23 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is TSA Vivid 520?
- TSA Vivid™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.
- What laser excites TSA Vivid 520?
- TSA Vivid 520 is optimally excited by the 488 laser line. Its excitation maximum is 494 nm.