About FITC

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Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

Alternative Dyes to FITC

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Oregon Green 488 KIRAVIA Blue 520 ATTO 488 Oyster 488 PromoFluor 488P STAR 488 SureLight 488 VRDye 490 Chromeo 505 FITC Fluorescein MaxLight 490 NL 493 Chromeo 488 CoraLite Plus 488 CF488A Rhodamine 110

Dyes similar to FITC based on emission

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Acridine Orange Alexa Fluor 488 ATTO 488 BactoView Live Green BCECF (525em) pH9.0 BCECF AM BCECF BCECF (Red em) pH5.2 BODIPY 500/510 Calcium Green CellBrite Steady 488 CellROX Green CellTrace CFSE CellTrace Oregon Green CellTracker Violet CF503R CFSE Chromeo 488 Chromeo 505 Citrine FITC Plus CytoTrack Green DCF DMAO DyLight 488 eFluor 520 Fix Viability EvaGreen EvaGreen Plus ExoBrite 490/515 EV Membrane Stain eYFP Flash Phalloidin Green 488 Fluo-3 AM Fluo-3 Fluo-4 GelGreen GOLGI-ID Green HiLyte 488 iFluor 488 Kaede (green) KikGR1 (green) KIRAVIA Blue 520 Krome Orange LIVE 510 Live/Dead Fix Aqua Live/Dead Fix Green Live-or-Dye 488/515 LysoView 488 MagDot 525 Mag-Fluo-4 mAmetrine mCitrine MemBrite Fix 488/515 MitoLite Green EX488 MitoView Green MultiDot 525 Newport Green DCF NovaFluor Blue 530 Nuclear Green Nuclear-ID Green NucSpot Live 488 NucView 488 Oregon Green 488 BAPTA Oregon Green 488 Oregon Green 514 Oyster 488 PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue ROS-490, Total Oxidative Stress Indicator pHrodo Green Premo Cameleon Calcium Sensor (Ca2+ bound) Premo Cameleon Calcium Sensor (Ca2+ free) PromoFluor 488P Qdot 525 ReadiLink 492-516 Rhodamine 110 Rhodamine 123 Rhodamine Green Riboflavin ROS-ID Green Spectrum Green STAR 488 STAR 512 STAR GREEN SureLight 488 SYBR Green Syto 16 Sytox Green TFAX 488 SE TFAX 488 TFP Thiazole Green Thiazole Orange Thiazole Orange Homodimer Thiazole Orange Iodide Topaz TO-Pro 1 TOTO-1 TSA Vivid 520 Venus ViaFluor 488 Viobility 405/520 Fixable Dye Viobility 488/520 Fixable Dye Vio Bright FITC Vio Green Vybrant DyeCycle Green YFP ZsGreen ABflo 488 ATTO 495 ATTO Rho110 XFD488 RayBright Violet 500 AbBy Fluor 488 Mpro Tracer