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Vio Green

Vio®Green (Ex-Max 388 nm/Em-Max 520 nm)

Vio®Green from Miltenyi has an excitation peak at 388 nm and an emission peak at 520 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
388 nm
Emission maximum
520 nm
Optimal laser line
405
Optimal filter
525/50
Relative brightness
1 of 5
Commercial antibodies
316 from 1 supplier

Vio Green has an excitation maximum at 388 nm and an emission maximum at 520 nm, a Stokes shift of 132 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is Vio Green?
Vio®Green from Miltenyi has an excitation peak at 388 nm and an emission peak at 520 nm.
What laser excites Vio Green?
Vio Green is optimally excited by the 405 laser line. Its excitation maximum is 388 nm.
What filter should I use to detect Vio Green?
A 525/50 filter captures the peak of the Vio Green emission near 520 nm with minimal spillover.
How bright is Vio Green?
Vio Green has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to Vio Green?
FluoroFinder tracks 316 commercially available Vio Green conjugates, searchable by target, clone, host and application.