DCF
DCF (Ex-Max 501 nm/Em-Max 524 nm)
Dichlorofluorescein (DCF) is an oxidation-sensitive organic probe used to detect the prescence of ROS (reactive oxygen species). Upon oxidation, it has an excitation peak at 501 nm and an emission peak at 524.
Excitation & emission spectra
Key facts
- Excitation maximum
- 501 nm
- Emission maximum
- 524 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Commercial antibodies
- 2 from 2 suppliers
DCF has an excitation maximum at 501 nm and an emission maximum at 524 nm, a Stokes shift of 23 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is DCF?
- Dichlorofluorescein (DCF) is an oxidation-sensitive organic probe used to detect the prescence of ROS (reactive oxygen species). Upon oxidation, it has an excitation peak at 501 nm and an emission peak at 524.
- What laser excites DCF?
- DCF is optimally excited by the 488 laser line. Its excitation maximum is 501 nm.
- What filter should I use to detect DCF?
- A 525/50 filter captures the peak of the DCF emission near 524 nm with minimal spillover.
- How many antibodies are available conjugated to DCF?
- FluoroFinder tracks 2 commercially available DCF conjugates, searchable by target, clone, host and application.