CFSE
CFSE (Ex-Max 494 nm/Em-Max 521 nm)
Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a green-emitting amine-reactive dye that can be excited by the 488 nm blue laser and captured using a 530/43 bandpass filter. It has excitation peak at 494 nm and and an emission peak at 521 nm. CFSE is most commonly used for in vivo cell tracking and proliferation studies. When a cell reproduces, the dye is distributed evenly to daughter cells, with fluorescence intensity halving with each successive passage. This is a generic dye and it can be found in kits from various suppliers.
Excitation & emission spectra
Key facts
- Excitation maximum
- 494 nm
- Emission maximum
- 521 nm
- Optimal laser line
- 488
- Optimal filter
- 530/43
CFSE has an excitation maximum at 494 nm and an emission maximum at 521 nm, a Stokes shift of 27 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CFSE?
- Carboxyfluorescein diacetate succinimidyl ester (CFSE) is a green-emitting amine-reactive dye that can be excited by the 488 nm blue laser and captured using a 530/43 bandpass filter. It has excitation peak at 494 nm and and an emission peak at 521 nm. CFSE is most commonly used for in vivo cell tracking and proliferation studies. When a cell reproduces, the dye is distributed evenly to daughter cells, with fluorescence intensity halving with each successive passage. This is a generic dye and it can be found in kits from various suppliers.
- What laser excites CFSE?
- CFSE is optimally excited by the 488 laser line. Its excitation maximum is 494 nm.
- What filter should I use to detect CFSE?
- A 530/43 filter captures the peak of the CFSE emission near 521 nm with minimal spillover.