Loading the Dye Directory…

Nuclear Green

Nuclear Green (Ex-Max 505 nm/Em-Max 530 nm)

Nuclear Green from AAT Bioquest is a cell-permeant, fluorogenic DNA binding dye that is excited at 503 nm and emits at 527 nm. It is used in the following applications: Flow Cytometry, Fluorescence Microscopy.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
505 nm
Emission maximum
530 nm
Optimal laser line
488
Optimal filter
515/30

Nuclear Green has an excitation maximum at 505 nm and an emission maximum at 530 nm, a Stokes shift of 25 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is Nuclear Green?
Nuclear Green from AAT Bioquest is a cell-permeant, fluorogenic DNA binding dye that is excited at 503 nm and emits at 527 nm. It is used in the following applications: Flow Cytometry, Fluorescence Microscopy.
What laser excites Nuclear Green?
Nuclear Green is optimally excited by the 488 laser line. Its excitation maximum is 505 nm.
What filter should I use to detect Nuclear Green?
A 515/30 filter captures the peak of the Nuclear Green emission near 530 nm with minimal spillover.